evaluating the effect of dracocephalum kotschyi methanol extract on mycobacterium tuberculosis

نویسندگان

g. asghari

b. nasr esfahani

p. paydar

چکیده

background and objectives: tuberculosis (tb) is the major public health problem in the world. each year there are 2-3 million deaths worldwide caused by tb. the increasing incidence of multi drug resistance tuberculosis (mdr-tb) worldwide highlights the urgent need to search for new anti-tuberculosis compounds. it has been reported that medicinal plant, dracocephalum kotschyi, possesses some antibacterial effect, thus in the present study its anti-mycobacterial property was evaluated. methods: the sensitivity and resistance of m. tuberculosis strains at concentration of 0.2 µg/ml isoniazid was determined by proportion method. methanol extract of d. kotschyi was prepared using maceration method. six concentrations of d. kotschyi, including 20, 40, 80, 160, 320 and 640μg/ml were prepared and its anti-mycobacterial effect on four groups of m. tuberculosis including m. tuberculosis h37rv (atcc 27294), isoniazid susceptible and resistance and mdr strains was determined. results: the methanol extract of d. kotschyi was significantly against m. tuberculosis. the percent of growthwas decreased from 100% to 0% in m. tuberculosis  h37rv (atcc 27294),  isoniazid resistant and isoniazid susceptible strains but from 100% to 50% in mdr strain in 640 μg/ml concentration. conclusion: the results showed that different concentrations of d. kotschyi methanol extracts showed a remarkable inhibitory effect on m. tuberculosis. identification of the effective fraction of d. kotschyi against m. tuberculosis is a further step to be studied.

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Evaluating the effect of Dracocephalum kotschyi methanol extract on Mycobacterium tuberculosis

Background and objectives: Tuberculosis (TB) is the major public health problem in the world. Each year there are 2-3 million deaths worldwide caused by TB. The increasing incidence of Multi Drug Resistance tuberculosis (MDR-TB) worldwide highlights the urgent need to search for new anti-tuberculosis compounds. It has been reported that medicinal plant, Dracocephalum kotschyi, possesse...

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عنوان ژورنال:
research journal of pharmacognosy

ناشر: the iranian society of pharmacognosy

ISSN 2345-4458

دوره 2

شماره 3 2015

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